Hydrogen sulfide regulates muscle RING finger-1 protein S-sulfhydration at Cys⁴⁴ to prevent cardiac structural damage in diabetic cardiomyopathy.

ABSTRACT:

Background and purpose

Hydrogen sulfide (H₂ S) plays important roles as a gasotransmitter in pathologies. Increased expression of the E3 ubiquitin ligase, muscle RING finger-1 (MuRF1), may be involved in diabetic cardiomyopathy. Here we have investigated whether and how exogenous H₂ S alleviates cardiac muscle degradation through modifications of MuRF1 S-sulfhydration in db/db mice.

Experimental approach

Neonatal rat cardiomyocytes were treated with high glucose (40 mM), oleate (100 μM), palmitate (400 μM), and NaHS (100 μM) for 72 hr. MuRF1 was silenced with siRNA technology and mutation at Cys⁴⁴ . Endoplasmic reticulum stress markers, MuRF1 expression, and ubiquitination level were measured. db/db mice were injected with NaHS (39 μmol·kg

SUBMITTER: Sun X 

PROVIDER: S-EPMC7024715 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature