Exploring the Mesenchymal Stem Cell Secretome for Corneal Endothelial Proliferation.

ABSTRACT: Ex vivo grown human corneal endothelial cells (HCEnC) are a new emerging treatment option to treat visually impaired patients aimed at alleviating the current global donor shortage. Expanding HCEnC is still challenging, and obtaining cells in sufficient quantities is a limiting factor. It is already known that conditioned medium obtained from bone marrow mesenchymal stem cells can stimulate the proliferation of endothelial cells. The aim of this study was to take this work a step further to identify some of the underlying factors responsible. We confirmed the stimulatory effect of the mesenchymal stem cell secretome seen previously and separated the exosomes from the soluble proteins using size exclusion chromatography. We demonstrated the presence of exosomes and soluble proteins in the e

SUBMITTER: Van den Bogerd B 

PROVIDER: S-EPMC7025074 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature