Systematic genetics and single-cell imaging reveal widespread morphological pleiotropy and cell-to-cell variability.

ABSTRACT: Our ability to understand the genotype-to-phenotype relationship is hindered by the lack of detailed understanding of phenotypes at a single-cell level. To systematically assess cell-to-cell phenotypic variability, we combined automated yeast genetics, high-content screening and neural network-based image analysis of single cells, focussing on genes that influence the architecture of four subcellular compartments of the endocytic pathway as a model system. Our unbiased assessment of the morphology of these compartments-endocytic patch, actin patch, late endosome and vacuole-identified 17 distinct mutant phenotypes associated with ~1,600 genes (~30% of all yeast genes). Approximately half of these mutants exhibited multiple phenotypes, highlighting the extent of morphological pleiotropy. Qu

SUBMITTER: Mattiazzi Usaj M 

PROVIDER: S-EPMC7025093 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature