Characterization of a reproducible model of fracture healing in mice using an open femoral osteotomy.

ABSTRACT:

Purpose

The classic fracture model, described by Bonnarens and Einhorn in 1984, enlists a blunt guillotine to generate a closed fracture in a pre-stabilized rodent femur. However, in less experienced hands, this technique yields considerable variability in fracture pattern and requires highly-specialized equipment. This study describes a reproducible and low-cost model of mouse fracture healing using an open femoral osteotomy.

Methods

Femur fractures were produced in skeletally mature male and female mice using an open femoral osteotomy after intramedullary stabilization. Mice were recovered for up to 28 days prior to analysis with microradiographs, histomorphometry, a novel μCT methodology, and biomechanical torsion testing at weekly intervals.

Results

Eight mice wer

SUBMITTER: Collier CD 

PROVIDER: S-EPMC7025178 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature