MTOR may interact with PARP-1 to regulate visible light-induced parthanatos in photoreceptors.

ABSTRACT:

Background

Excessive light exposure is a detrimental environmental factor that plays a critical role in the pathogenesis of retinal degeneration. However, the mechanism of light-induced death of retina/photoreceptor cells remains unclear. The mammalian/mechanistic target of rapamycin (mTOR) and Poly (ADP-ribose) polymerase-1 (PARP-1) have become the primary targets for treating many neurodegenerative disorders. The aim of this study was to elucidate the mechanisms underlying light-induced photoreceptor cell death and whether the neuroprotective effects of mTOR and PARP-1 inhibition against death are mediated through apoptosis-inducing factor (AIF).

Methods

Propidium iodide (PI)/Hoechst staining, lentiviral-mediated short hairpin RNA (shRNA), Western blot analysis, cellular f

SUBMITTER: Pan YR 

PROVIDER: S-EPMC7025415 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature