Integrated analysis of directly captured microRNA targets reveals the impact of microRNAs on mammalian transcriptome.

ABSTRACT: MicroRNA (miRNA)-mediated regulation is widespread, relatively mild but functionally important. It remains challenging to unequivocally identify miRNA targeted RNAs at a genomic scale and determine how changes in miRNA levels affect the transcriptome. Here, we captured individual miRNAs and their targeted RNA sites in wild-type, miR-200 family knockout and induced epithelial cells. We detected 1797 miRNAs interacting with 13,830 transcripts at 616,127 sites by sequencing 1,230,019 unique miRNA:RNA chimeras. Although mRNA sites that are bound by miRNAs and contain matches to seed sequences confer the strongest regulation, ∼40%-60% of miRNA bound regions do not contain seed matches. Different miRNAs have different preferences to seed matches and 3' end base-pairing. For individual miRNAs, th

SUBMITTER: Bjerke GA 

PROVIDER: S-EPMC7025510 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature