Secretome Analysis Performed During in vitro Cardiac Differentiation: Discovering the Cardiac Microenvironment.

ABSTRACT: Human pluripotent stem cells are an important tool for the study of developmental processes, such as cardiomyogenic differentiation. Despite the advances made in this field, the molecular and cellular signals involved in the commitment of embryonic stem cells to the cardiac phenotype are still under investigation. Therefore, this study focuses on identifying the extracellular signals involved in in vitro cardiac differentiation of human embryonic stem cells. Using a three-dimensional cardiomyogenic differentiation protocol, the conditioned medium and the extracellular matrix (ECM) of embryoid body cultures were collected and characterized at four specific time points. Mass spectrometry (MS) and antibody array analysis of the secretome identified a number of secreted proteins related

SUBMITTER: Robert AW 

PROVIDER: S-EPMC7025591 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature