Application of the direct peptide reactivity assay (DPRA) to inorganic compounds: a case study of platinum species.

ABSTRACT: The in chemico Direct Peptide Reactivity Assay (DPRA) was developed as a non-animal, relatively high throughput, screening tool for skin sensitization potential. Although the Adverse Outcome Pathway (AOP) for respiratory sensitization remains to be fully elucidated, it is recognized that the molecular initiation event for both skin and respiratory sensitization to low molecular weight chemicals involves haptenation with proteins. The DPRA examines the reactivity of a test compound to two model peptides (containing either cysteine or lysine) and consequently is able to screen for both skin and respiratory sensitization potential. The DPRA was primarily developed for and validated with organic compounds and assessment of the applicability of the assay to metal compounds has received only lim

SUBMITTER: Hemming JDC 

PROVIDER: S-EPMC7025741 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature