YTHDF2 Recognition of N¹-Methyladenosine (m¹A)-Modified RNA Is Associated with Transcript Destabilization.

ABSTRACT: Epitranscriptomic modifications play an important role in RNA function and can impact gene expression. Here, we apply a chemical proteomics approach to investigate readers of N¹-methyladenosine (m¹A), a poorly characterized modification on mammalian mRNA. We find that YTHDF proteins, known m⁶A readers, recognize m¹A-modified sequences in a methylation-specific manner. We characterize binding of recombinant YTHDF1/2 proteins to m¹A-modified oligonucleotides to demonstrate that these interactions can exhibit comparable affinity to m⁶A-recognition events and occur in diverse sequence contexts. Further, we demonstrate YTHDF2 interacts specifically with endogenously modified m¹A transcripts. Finally, we deplete cellula

SUBMITTER: Seo KW 

PROVIDER: S-EPMC7025767 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature