Bacterial interspecies interactions modulate pH-mediated antibiotic tolerance.

ABSTRACT: Predicting antibiotic efficacy within microbial communities remains highly challenging. Interspecies interactions can impact antibiotic activity through many mechanisms, including alterations to bacterial physiology. Here, we studied synthetic communities constructed from the core members of the fruit fly gut microbiota. Co-culturing of Lactobacillus plantarum with Acetobacter species altered its tolerance to the transcriptional inhibitor rifampin. By measuring key metabolites and environmental pH, we determined that Acetobacter species counter the acidification driven by L. plantarum production of lactate. Shifts in pH were sufficient to modulate L. plantarum tolerance to rifampin and the translational inhibitor erythromycin. A reduction in lag time exit

SUBMITTER: Aranda-Diaz A 

PROVIDER: S-EPMC7025823 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature