Mkt1 is required for RNAi-mediated silencing and establishment of heterochromatin in fission yeast.

ABSTRACT: Constitutive domains of repressive heterochromatin are maintained within the fission yeast genome through self-reinforcing mechanisms involving histone methylation and small RNAs. Non-coding RNAs generated from heterochromatic regions are processed into small RNAs by the RNA interference pathway, and are subject to silencing through both transcriptional and post-transcriptional mechanisms. While the pathways involved in maintenance of the repressive heterochromatin state are reasonably well understood, less is known about the requirements for its establishment. Here, we describe a novel role for the post-transcriptional regulatory factor Mkt1 in establishment of heterochromatin at pericentromeres in fission yeast. Loss of Mkt1 does not affect maintenance of existing heterochromatin, but do

SUBMITTER: Taglini F 

PROVIDER: S-EPMC7026591 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature