Subtle structural alterations in G-quadruplex DNA regulate site specificity of fluorescence light-up probes.

ABSTRACT: G-quadruplex (G4) DNA structures are linked to key biological processes and human diseases. Small molecules that target specific G4 DNA structures and signal their presence would therefore be of great value as chemical research tools with potential to further advance towards diagnostic and therapeutic developments. However, the development of these types of specific compounds remain as a great challenge. In here, we have developed a compound with ability to specifically signal a certain c-MYC G4 DNA structure through a fluorescence light-up mechanism. Despite the compound's two binding sites on the G4 DNA structure, only one of them result in the fluorescence light-up effect. This G-tetrad selectivity proved to originate from a difference in flexibility that affected the binding affinity a

SUBMITTER: Kumar R 

PROVIDER: S-EPMC7026600 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature