Dynamic changes in intron retention are tightly associated with regulation of splicing factors and proliferative activity during B-cell development.

ABSTRACT: Intron retention (IR) has been proposed to modulate the delay between transcription and translation. Here, we provide an exhaustive characterization of IR in differentiated white blood cells from both the myeloid and lymphoid lineage where we observed highest levels of IR in monocytes and B-cells, in addition to previously reported granulocytes. During B-cell differentiation, we found an increase in IR from the bone marrow precursors to cells residing in secondary lymphoid organs. B-cells that undergo affinity maturation to become antibody producing plasma cells steadily decrease retention. In general, we found an inverse relationship between global IR levels and both the proliferative state of cells, and the global levels of expression of splicing factors. IR dynamics during B-cell differ

SUBMITTER: Ullrich S 

PROVIDER: S-EPMC7026658 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature