Cell type-specific mechanisms coupling protease-activated receptor-1 to infectious colitis pathogenesis.

ABSTRACT:

Background

Protease-activated receptor-1 (PAR-1) plays a major role in multiple disease processes, including colitis. Understanding the mechanisms coupling PAR-1 to disease pathogenesis is complicated by the fact that PAR-1 is broadly expressed across multiple cell types.

Objective

Determine the specific contributions of PAR-1 expressed by macrophages and colonic enterocytes to infectious colitis.

Methods

Mice carrying a conditional PAR-1 allele were generated and bred to mice expressing Cre recombinase in a myeloid- (PAR-1^ΔM ) or enterocyte-specific (PAR-1^ΔEPI ) fashion. Citrobacter rodentium colitis pathogenesis was analyzed in mice with global PAR-1 deletion (PAR-1^-/- ) and cell type-specific deletions.

Results

Constitutive d

SUBMITTER: Boucher AA 

PROVIDER: S-EPMC7026906 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature