Evaluation of RNA purification methods by using different blood stabilization tubes: identification of key features for epidemiological studies.

ABSTRACT: OBJECTIVE:Peripheral blood is the most promising source of RNA biomarkers for diagnostic and epidemiological studies, because the presence of disease and prognostic information is reflected in the gene expression pattern. Quality RNA is used by a number of different downstream applications, so the selection of the most appropriate RNA stabilization and purification method is important. We have analyzed the RNA purified from 300 blood samples from 25 donors processed by two technicians using three methodologies with Tempus and PaxGene tubes. RESULTS:The best quality sample results were obtained with the Tempus Spin RNA Isolation Kit and the PaxGene Blood miRNA Kit, although larger amounts of RNA were obtained with the Tempus Spin RNA Isolation Kit. Lower Cq values were observed for RNA and

SUBMITTER: Carrillo-Avila JA 

PROVIDER: S-EPMC7026973 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature