Engineering yeast artificial core promoter with designated base motifs.

ABSTRACT:

Background

Synthetic biology requires toolbox of promoters to finely tune gene expression levels for building up efficient cell factories. Yeast promoters owned variable core promoter regions between the TATA-box and transcriptional starting site (TSS) at the length mostly around 20-80 bases. This region allowed flexible design of artificial promoter but potentially demand special base motifs to maintain or enhance the promoter's strength.

Results

Here, we designed and screened the base motifs and tested the activities of yeast artificial core promoters. Different 30 bases of artificial sequences led to variable expression levels of CrtY enzyme which determined the lycopene-carotene compositions, represented in the colony-color spectrum of red-orange-yellow. The upstream seq

SUBMITTER: Liu R 

PROVIDER: S-EPMC7026997 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature