Identification of universal and cell-type specific p53 DNA binding.

ABSTRACT:

Background

The tumor suppressor p53 is a major regulator of the DNA damage response and has been suggested to selectively bind and activate cell-type specific gene expression programs. However recent studies and meta-analyses of genomic data propose largely uniform, and condition independent p53 binding and thus question the selective and cell-type dependent function of p53.

Results

To systematically assess the cell-type specificity of p53, we measured its association with DNA in 12 p53 wild-type cancer cell lines, from a range of epithelial linages, in response to ionizing radiation. We found that the majority of bound sites were occupied across all cell lines, however we also identified a subset of binding sites that were specific to one or a few cell lines. Unlike the sha

SUBMITTER: Hafner A 

PROVIDER: S-EPMC7027055 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature