A simple monochromatic flow cytometric assay for assessment of intraerythrocytic development of Plasmodium falciparum.

ABSTRACT: BACKGROUND:Gold standard microscopic examination of Plasmodium falciparum intraerythrocytic stage remains an important process for staging and enumerating parasitized erythrocytes in culture; however, microscopy is laborious and its accuracy is dependent upon the skill of the examiner. METHODS:In this study, ViSafe Green (VSG), which is a nucleic acid-binding fluorescent dye, was used for assessing in vitro development of P. falciparum using flow cytometry. RESULTS:Fluorescence intensity of VSG was found to depend on the developmental stage of parasites. Specifically, multiple-nuclei-containing schizonts were observed in the VSGhigh population, and growing trophozoites and ring-shaped forms were observed in the VSGintermediate and VSGlow populations. The efficacy of VSG-based assay was fou

SUBMITTER: Kulkeaw K 

PROVIDER: S-EPMC7027058 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature