Keratinocyte footprint assay discriminates antilaminin-332 pemphigoid from all other forms of pemphigoid diseases.

ABSTRACT:

Background

Antilaminin-332 mucous membrane pemphigoid is a chronic severe pemphigoid disease characterized by autoantibodies to laminin-332. At present no commercial assay is available to demonstrate antilaminin-332 antibodies, and diagnosis relies on in-house techniques with limited sensitivities.

Objectives

In order to move, keratinocytes cultured in vitro secrete laminin-332 to attach to the culture dish. In that way, they leave behind a unique footprint trail of laminin-332. We aimed to develop a sensitive and specific laboratory assay to determine antilaminin-332 autoantibodies in patient serum based on binding of patient IgG to these unique footprints.

Methods

Normal human keratinocytes were grown on glass coverslips and incubated with patient or control serum f

SUBMITTER: Giurdanella F 

PROVIDER: S-EPMC7027452 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature