Interspecies differences in protein expression do not impact the spatiotemporal regulation of glycoprotein VI mediated activation.

ABSTRACT:

Background

Accurate protein quantification is a vital prerequisite for generating meaningful predictions when using systems biology approaches, a method that is increasingly being used to unravel the complexities of subcellular interactions and as part of the drug discovery process. Quantitative proteomics, flow cytometry, and western blotting have been extensively used to define human platelet protein copy numbers, yet for mouse platelets, a model widely used for platelet research, evidence is largely limited to a single proteomic dataset in which the total amount of proteins was generally comparatively higher than those found in human platelets.

Objectives

To investigate the functional implications of discrepancies between levels of mouse and human proteins in the glycopro

SUBMITTER: Dunster JL 

PROVIDER: S-EPMC7027541 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature