Two-photon microscopic observation of cell-production dynamics in the developing mammalian neocortex in utero.

ABSTRACT: Morphogenesis and organ development should be understood based on a thorough description of cellular dynamics. Recent studies have explored the dynamic behaviors of mammalian neural progenitor cells (NPCs) using slice cultures in which three-dimensional systems conserve in vivo-like environments to a considerable degree. However, live observation of NPCs existing truly in vivo, as has long been performed for zebrafish NPCs, has yet to be established in mammals. Here, we performed intravital two-photon microscopic observation of NPCs in the developing cerebral cortex of H2B-EGFP or Fucci transgenic mice in utero. Fetuses in the uterine sac were immobilized using several devices and were observed through a window made in the uterine wall and the amniotic membrane while monitoring blood circu

SUBMITTER: Kawasoe R 

PROVIDER: S-EPMC7027555 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature