Evolutionary adaptation of Aspergillus niger for increased ferulic acid tolerance.

ABSTRACT:

Aims

To create an Aspergillus niger mutant with increased tolerance against ferulic acid using evolutionary adaptation.

Methods and results

Evolutionary adaptation of A. niger N402 was performed by consecutive growth on increasing concentrations of ferulic acid in the presence of 25 mmol l^-1 d-fructose, starting from 0·5 mmol l^-1 and ending with 5 mmol l^-1 ferulic acid. The A. niger mutant obtained after six months, named Fa6, showed increased ferulic acid tolerance compared to the parent. In addition, Fa6 has increased ferulic acid consumption and a higher conversion rate, suggesting that the mutation affects aromatic metabolism of this species. Transcriptome analysis of the evolutionary mutant on ferulic acid revealed a distinct gene expres

SUBMITTER: Lubbers RJM 

PROVIDER: S-EPMC7027748 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature