Epitranscriptomic profiling in human placenta: N6-methyladenosine modification at the 5'-untranslated region is related to fetal growth and preeclampsia.

ABSTRACT: Intracellular mRNA levels are not always proportional to their respective protein levels, especially in the placenta. This discrepancy may be attributed to various factors including post-transcriptional regulation, such as mRNA methylation (N6-methyladenosine: m⁶A). Here, we conducted a comprehensive m⁶A analysis of human placental tissue from neonates with various birth weights to clarify the involvement of m⁶A in placental biology. The augmented m⁶A levels at the 5'-untranslated region (UTR) in mRNAs of small-for-date placenta samples were dominant compared to reduction of m⁶A levels, whereas a decrease in m⁶A in the vicinity of stop codons was common in heavy-for-date placenta samples. Notably, most of these genes showed

SUBMITTER: Taniguchi K 

PROVIDER: S-EPMC7027905 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature