Development and characterization of a continuous cell line (EL) from the liver of European eel Anguilla anguilla.

ABSTRACT: In the present study, a new hepatic tissue-origin cell line from European eel Anguilla anguilla has been developed and characterized. This cell line designated EL has been maintained in Leibovitz L-15 supplemented with 10% fetal bovine serum over 72 months, and subcultured more than 90 times. The EL cell line consisted predominantly of fibroblast-like cells, which could survive over 100 days in vitro, and could grow at 15-32°C. The optimum temperature for growth was 27°C. The chromosome analysis revealed a modal diploid karyotype of 2n = 38. The origin of this cell line was confirmed by the 18S recombinant (r)RNA sequencing. The susceptibility test indicated significant cytopathic effects in the EL cells with regard to the Rana grylio virus and the Herpesvirus anguillae. The viral replicat

SUBMITTER: Zheng Z 

PROVIDER: S-EPMC7028054 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature