A Novel Strategy for Enhance Potentiation of Meglumine antimoniate against Leishmania major In Vitro.

ABSTRACT:

Background

We aimed to design a different method of drug delivery for increased transfer of the choice drug (meglumine antimoniate) within the host cells. Therefore, listeriolysin O (LLO), a bacterial product which is a member of pore-forming peptides was used as an enhancer factor with meglumine antimoniate in order to facilitate the transition of the drug across macrophage membrane.

Methods

LLO was produced in Isfahan University of Medical Sciences in 2016, by expressing the hlyA gene in Escherichia coli and purified using affinity chromatography. Cytotoxicity of the purified protein was investigated in an in vitro model of macrophage Leishmania infection.

Results

LLO was cytotoxic against murine macrophage cells (J774-A1) and amastigote forms o

SUBMITTER: Mirzaei F 

PROVIDER: S-EPMC7028224 | biostudies-literature | 2019 Oct-Dec

REPOSITORIES: biostudies-literature