Maximizing precision and accuracy of the doubly labeled water method via optimal sampling protocol, calculation choices, and incorporation of ¹⁷O measurements.

ABSTRACT:

Background/objectives

The doubly labeled water (DLW) method is the gold standard methodology for determination of free-living, total daily energy expenditure (TEE). However, there is no single accepted approach for either the sampling protocols (daily vs. two-point, in which samples are collected after dosing and at the end of the measurement period) or the calculations used in the determination of the rate of carbon dioxide production (rCO₂) and TEE. Moreover, fluctuations in natural background abundances introduce error in the calculation of rCO₂ and TEE. The advent of new technologies makes feasible the possibility of including additional isotope measures (¹⁷O) to account for background variation, which may improve accuracy.

Subjects/methods</

SUBMITTER: Berman ESF 

PROVIDER: S-EPMC7028494 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature