Red fluorescent CEPIA indicators for visualization of Ca²⁺ dynamics in mitochondria.

ABSTRACT: Mitochondrial Ca²⁺ dynamics are involved in the regulation of multifarious cellular processes, including intracellular Ca²⁺ signalling, cell metabolism and cell death. Use of mitochondria-targeted genetically encoded Ca²⁺ indicators has revealed intercellular and subcellular heterogeneity of mitochondrial Ca²⁺ dynamics, which are assumed to be determined by distinct thresholds of Ca²⁺ increases at each subcellular mitochondrial domain. The balance between Ca²⁺ influx through the mitochondrial calcium uniporter and extrusion by cation exchangers across the inner mitochondrial membrane may define the threshold; however, the precise mechanisms remain to be further explored. We here report the new red fluorescent genetically enc

SUBMITTER: Kanemaru K 

PROVIDER: S-EPMC7029041 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature