Transient Cell Membrane Disruptions induce Calcium Waves in Corneal Keratocytes.

ABSTRACT: The purpose of this study was to determine if transient cell membrane disruptions (TPMDs) in single keratocytes can trigger signaling events in neighboring keratocytes. Stromal cells were cultured from human corneas (HCSC) and mouse corneas (MCSC). TPMDs were produced using a multiphoton microscope in Cal-520-AM loaded cells. TPMD-induced calcium increases (Ca⁺⁺_i) were measured in Ca⁺⁺-containing and Ca⁺⁺-free solutions containing thapsigargin, ryanodine, BAPTA-AM, 18-α-glycyrrhetinic acid (18α-GA), apyrase, BCTC, AMG 9810, or AMTB. Fluorescence intensity was recorded as the number of cells responding and the area under the fluorescence versus time curve. The maximum distance of responding neighboring cells in ex vivo human corneas was measured.

SUBMITTER: Chen Z 

PROVIDER: S-EPMC7029045 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature