The acute effects of growth hormone in adipose tissue is associated with suppression of antilipolytic signals.

ABSTRACT:

Aim

Since GH stimulates lipolysis in vivo after a 2-hr lag phase, we studied whether this involves GH signaling and gene expression in adipose tissue (AT).

Methods

Human subjects (n = 9) each underwent intravenous exposure to GH versus saline with measurement of serum FFA, and GH signaling, gene array, and protein in AT biopsies after 30-120 min. Human data were corroborated in adipose-specific GH receptor knockout (FaGHRKO) mice versus wild-type mice. Expression of candidate genes identified in the array were investigated in 3T3-L1 adipocytes.

Results

GH increased serum FFA and AT phosphorylation of STAT5b in human subjects. This was replicated in wild-type mice, but not in FaGHRKO mice. The array identified 53 GH-regulated genes, and Ingenuity Pathway analysis showe

SUBMITTER: Hoyer KL 

PROVIDER: S-EPMC7029434 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature