Conditioned medium derived from FGF-2-modified GMSCs enhances migration and angiogenesis of human umbilical vein endothelial cells.

ABSTRACT:

Background

Angiogenesis plays an important role in tissue repair and regeneration, and conditioned medium (CM) derived from mesenchymal stem cells (MSC-CM) possesses pro-angiogenesis. Nevertheless, the profile and concentration of growth factors in MSC-CM remain to be optimized. Fibroblast growth factor-2 (FGF-2) has been proven to be an effective angiogenic factor. Thus, the aim of this study was to verify whether FGF-2 gene overexpression optimized CM from human gingival mesenchymal stem cells (hGMSCs) and whether such optimized CM possessed more favorable pro-angiogenesis effect.

Methods

First, FGF-2 gene-modified hGMSCs were constructed using lentiviral transfection technology (LV-FGF-2⁺-hGMSCs) and the concentration of angiogenesis-related factors in LV-FGF-2

SUBMITTER: Jin S 

PROVIDER: S-EPMC7029497 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature