Outer Radial Glia-like Cancer Stem Cells Contribute to Heterogeneity of Glioblastoma.

ABSTRACT: Glioblastoma is a devastating form of brain cancer. To identify aspects of tumor heterogeneity that may illuminate drivers of tumor invasion, we created a glioblastoma tumor cell atlas with single-cell transcriptomics of cancer cells mapped onto a reference framework of the developing and adult human brain. We find that multiple GSC subtypes exist within a single tumor. Within these GSCs, we identify an invasive cell population similar to outer radial glia (oRG), a fetal cell type that expands the stem cell niche in normal human cortex. Using live time-lapse imaging of primary resected tumors, we discover that tumor-derived oRG-like cells undergo characteristic mitotic somal translocation behavior previously only observed in human development, suggesting a reactivation of developmental pro

SUBMITTER: Bhaduri A 

PROVIDER: S-EPMC7029801 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature