[Effect of clock gene PER1 knockdown on clock gene networks in human oral squamous cell carcinoma].

ABSTRACT:

Objective

This study investigated the effect of clock gene PER1 on the expression levels of other clock genes in clock gene networks in oral squamous cell carcinoma cells.

Methods

We used RNA interference mediated by short hairpin RNAs (shRNAs) to effectively knock down PER1 in SCC15 human oral squamous cell carcinoma cells. Flow cytometry was used to detect the degree of proliferation and apoptosis of the cells after PER1 knockdown, and quantitative real-time PCR was used to detect the mRNA expression levels of the clock genes CLOCK, BMAL1, PER1, PER2, PER3, DEC1, DEC2, CRY1, CRY2, TIM, CKIE, RORA, NPAS2, and REV-ERBA.

Results

The proliferation index of SCC15 cells increased significantly while the apoptotic index decreased significantly after PER1 knockdown (P<0.05)

SUBMITTER: Qin Z 

PROVIDER: S-EPMC7030199 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature