Coexistence of Two bla _{NDM- 5} Genes Carried on IncX3 and IncFII Plasmids in an Escherichia coli Isolate Revealed by Illumina and Nanopore Sequencing.

ABSTRACT: The emergence of carbapenem-resistant Enterobacteriaceae poses a significant threat to public health worldwide. Here, we reported a multidrug-resistant Escherichia coli strain with two different bla _{NDM- 5}-carrying plasmids from China. Illumina short-read and MinION long-read whole genome sequencing were performed. Genomic analysis found that one bla _{NDM- 5} gene together with mphA was located on a 55-kb IncX3 plasmid, while the other bla _{NDM- 5} gene was on a novel 68-kb IncFII plasmid. Susceptibility testing and quantitative reverse transcription PCR results further indicated that the transconjugants with the IncX3 plasmid exhibited higher-level carbapenem resistance and expression o

SUBMITTER: Yang L 

PROVIDER: S-EPMC7031209 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature