Manipulating ATP supply improves in situ CO₂ recycling by reductive TCA cycle in engineered Escherichia coli.

ABSTRACT: The reductive tricarboxylic acid (rTCA) cycle was reconstructed in Escherichia coli by introducing pGETS118KAFS, where kor (encodes α-ketoglutarate:ferredoxin oxidoreductase), acl (encodes ATP-dependent citrate lyase), frd (encodes fumarate reductase), and sdh (encodes succinate dehydrogenase) were tandemly conjugated by the ordered gene assembly in Bacillus subtilis (OGAB). E. coli MZLF (E. coli BL21(DE3) Δzwf, Δldh, Δfrd) was employed so that the C-2/C-1 [(ethanol + acetate)/(formate + CO₂)] ratio can be used to investigate the effectiveness of the recombinant rTCA for in situ CO₂ recycling. It has been shown that supplying ATP through the energy pump (the EP), where formate donates electro

SUBMITTER: Chen CH 

PROVIDER: S-EPMC7031464 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature