Association of GCK gene DNA methylation with the risk of clopidogrel resistance in acute coronary syndrome patients.

ABSTRACT:

Backgrounds

Clopidogrel resistance (CR), which was manifested as the failure of platelet inhibition in clopidogrel treatment, was likely to lead to cardiovascular events. Our study was aimed to explore the contribution of DNA methylation in glucokinase (GCK) to the CR risk.

Methods

Among 36 CR and 36 non-CR acute coronary syndrome (ACS) patients, the platelet functions were evaluated by VerifyNow P2Y12 assay (turbidimetric-based optical detection) and DNA methylation levels on two fragments of the CGI from the GCK were investigated through bisulfite pyrosequencing methods. In addition, the GCK mRNA expression was analyzed via quantitative real-time PCR. Lastly, the logistic regression was employed to test the interaction between GCK methylation and nongenetic variables in CR

SUBMITTER: Su J 

PROVIDER: S-EPMC7031555 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature