Reprogramming of human fibroblasts into osteoblasts by insulin-like growth factor-binding protein 7.

ABSTRACT: The induced pluripotent stem cell (iPSC) is a promising cell source for tissue regeneration. However, the therapeutic value of iPSC technology is limited due to the complexity of induction protocols and potential risks of teratoma formation. A trans-differentiation approach employing natural factors may allow better control over reprogramming and improved safety. We report here a novel approach to drive trans-differentiation of human fibroblasts into functional osteoblasts using insulin-like growth factor binding protein 7 (IGFBP7). We initially determined that media conditioned by human osteoblasts can induce reprogramming of human fibroblasts to functional osteoblasts. Proteomic analysis identified IGFBP7 as being significantly elevated in media conditioned with osteoblasts compared with

SUBMITTER: Lu Z 

PROVIDER: S-EPMC7031646 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature