RP-HPLC and UV Spectrophotometric Analysis of Paracetamol, Ibuprofen, and Caffeine in Solid Pharmaceutical Dosage Forms by Derivative, Fourier, and Wavelet Transforms: A Comparison Study.

ABSTRACT: Different signal-transforming algorithms were applied for UV spectrophotometric analysis of paracetamol, ibuprofen, and caffeine in ternary mixtures. Phosphate buffer pH 7.2 was used as the spectrophotometric solvent. Severe overlapping spectra could be resolved into individual bands in the range of wavelengths 200-300 nm by using Savitzky-Golay smoothing and differentiation, trigonometric Fourier series, and mother wavelet functions (i.e., sym6, haar, coif3, and mexh). To optimize spectral recoveries, the concentration of various types of divisors (single, double, and successive) was tested. The developed spectrophotometric methods showed linearity over the ranges 20-40 mg/L for paracetamol, 12-32 mg/L for ibuprofen, and 1-3.5 mg/L for caffeine (R 2 > 0.990). They could be successfully ap

SUBMITTER: Vu Dang H 

PROVIDER: S-EPMC7031732 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature