Peroxisomal gene and protein expression increase in response to a high-lipid challenge in human skeletal muscle.

ABSTRACT: Peroxisomes are essential for lipid metabolism and disruption of liver peroxisomal function results in neonatal death. Little is known about how peroxisomal content and activity respond to changes in the lipid environment in human skeletal muscle (HSkM).

Aims

We hypothesized and tested that increased peroxisomal gene/protein expression and functionality occur in HSkM as an adaptive response to lipid oversupply.

Materials and methods

HSkM biopsies, derived from a total of sixty-two subjects, were collected for 1) examining correlations between peroxisomal proteins and intramyocellular lipid content (IMLC) as well as between peroxisomal functionality and IMLC, 2) assessing peroxisomal gene expression in response to acute- or 7-day high fat meal (HFM), and in human tissue derive

SUBMITTER: Huang TY 

PROVIDER: S-EPMC7031862 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature