Inhaled nitric oxide to control platelet hyper-reactivity in patients with acute submassive pulmonary embolism.

ABSTRACT:

Background

We test if inhaled nitric oxide (NO) attenuates platelet functional and metabolic hyper-reactivity in subjects with submassive pulmonary embolism (PE).

Methods

Participants with PE were randomized to either 50 ppm NO + O2 or O2 only for 24 h with blood sampling at enrollment and after treatment; results were compared with healthy controls. Platelet metabolic activity was assessed by oxygen consumption (basal and uncoupled) and reactivity was assessed with agonist-stimulated thromboelastography (TEG) and fluorometric measurement of agonist-stimulated cytosolic [Ca⁺⁺] without and with pharmacological soluble guanylate (sGC) modulation.

Results

Participants (N = 38 per group) were well-matched at enrollment for PE severity, comorbidities as well as

SUBMITTER: Kline JA 

PROVIDER: S-EPMC7032016 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature