Comparison of the performance of ITS1 and ITS2 as barcodes in amplicon-based sequencing of bioaerosols.

ABSTRACT: This paper presents the performance of two eukaryotic genomic ribosomal regions, ITS1 and ITS2, in describing fungal diversity in aerosol samples using amplicon-based High-Throughput Sequencing (HTS). Composting sites, biomethanization facilities, and dairy farms, all affected by the presence of fungi, were visited to collect air samples. The amplicon-based HTS approach is a target enrichment method that relies on the amplification of a specific target using particular primers before sequencing. Thus, the results are highly dependent on the quality of amplification. For this reason, the authors of this paper used a shotgun metagenomic approach to compare its outcome with the amplicon-based method. Indeed, shotgun metagenomic does not rely on any amplification prior to sequencing, because a

SUBMITTER: Mbareche H 

PROVIDER: S-EPMC7032056 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature