Human organotypic lymphatic vessel model elucidates microenvironment-dependent signaling and barrier function.

ABSTRACT: The lymphatic system is an active player in the pathogenesis of several human diseases, including lymphedema and cancer. Relevant models are needed to advance our understanding of lymphatic biology in disease progression to improve therapy and patient outcomes. Currently, there are few 3D in vitro lymphatic models that can recapitulate the physiological structure, function, and interactions of lymphatic vessels in normal and diseased microenvironments. Here, we developed a 3D microscale lymphatic vessel (μLYMPH) system for generating human lymphatic vessels with physiological tubular structure and function. Consistent with characteristics of lymphatic vessels in vivo, the endothelium of cultured vessels was leaky with an average permeability of 1.38 × 10^-5 ± 0.29 × 10^-5

SUBMITTER: Gong MM 

PROVIDER: S-EPMC7032699 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature