Giant Endoplasmic Reticulum vesicles (GERVs), a novel model membrane tool.

ABSTRACT: Artificial giant vesicles have proven highly useful as membrane models in a large variety of biophysical and biochemical studies. They feature accessibility for manipulation and detection, but lack the compositional complexity needed to reconstitute complicated cellular processes. For the plasma membrane (PM), this gap was bridged by the establishment of giant PM vesicles (GPMVs). These native membranes have facilitated studies of protein and lipid diffusion, protein interactions, electrophysiology, fluorescence analysis of lateral domain formation and protein and lipid partitioning as well as mechanical membrane properties and remodeling. The endoplasmic reticulum (ER) is key to a plethora of biological processes in any eukaryotic cell. However, its intracellular location and dynamic and

SUBMITTER: Grimmer M 

PROVIDER: S-EPMC7033103 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature