The effects of electroporation buffer composition on cell viability and electro-transfection efficiency.

ABSTRACT: Electroporation is an electro-physical, non-viral approach to perform DNA, RNA, and protein transfections of cells. Upon application of an electric field, the cell membrane is compromised, allowing the delivery of exogenous materials into cells. Cell viability and electro-transfection efficiency (eTE) are dependent on various experimental factors, including pulse waveform, vector concentration, cell type/density, and electroporation buffer properties. In this work, the effects of buffer composition on cell viability and eTE were systematically explored for plasmid DNA encoding green fluorescent protein following electroporation of 3T3 fibroblasts. A HEPES-based buffer was used in conjunction with various salts and sugars to modulate conductivity and osmolality, respectively. Pulse applicat

SUBMITTER: Sherba JJ 

PROVIDER: S-EPMC7033148 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature