Gintonin stimulates autophagic flux in primary cortical astrocytes.

ABSTRACT:

Background

Gintonin (GT), a novel ginseng-derived exogenous ligand of lysophosphatidic acid (LPA) receptors, has been shown to induce cell proliferation and migration in the hippocampus, regulate calcium-dependent ion channels in the astrocytes, and reduce β-amyloid plaque in the brain. However, whether GT influences autophagy in cortical astrocytes is not yet investigated.

Methods

We examined the effect of GT on autophagy in primary cortical astrocytes using immunoblot and immunocytochemistry assays. Suppression of specific proteins was performed via siRNA. LC3 puncta was determined using confocal microscopy.

Results

GT strongly upregulated autophagy marker LC3 by a concentration- as well as time-dependent manner via G protein-coupled LPA receptors. GT-induced autoph

SUBMITTER: Rahman MA 

PROVIDER: S-EPMC7033340 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature