High Glucose-Induced TRPC6 Channel Activation Decreases Glutamate Uptake in Rat Retinal Muller Cells.

ABSTRACT: High glucose (HG) increases the production of reactive oxygen species (ROS), leading to decreased glutamate uptake in Müller cells. The transient receptor potential cation channel 6 (TRPC6) channel, an oxidative stress-sensitive Ca²⁺-permeable cationic channel, is readily detected in Müller cells and highly expressed under HG conditions. Yet, the effect of high glucose-induced TRPC6 channel activation in Müller cells is poorly understood. We hypothesized that TRPC6 channel activation mediates high glucose-induced decreases in Müller cell glutamate uptake. We found RNA interference (RNAi) of the TRPC6 channel abolished HG-induced decreases in glutamate uptake and cell death. HG also decreased the expression of the glutamate-aspartate transporter (GLAST), which is the most importa

SUBMITTER: Ma M 

PROVIDER: S-EPMC7033573 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature