MBOAT7-driven phosphatidylinositol remodeling promotes the progression of clear cell renal carcinoma.

ABSTRACT:

Objective

The most common kidney cancer, clear cell renal cell carcinoma (ccRCC), is closely associated with obesity. The "clear cell" variant of RCC gets its name from the large lipid droplets that accumulate in the tumor cells. Although renal lipid metabolism is altered in ccRCC, the mechanisms and lipids driving this are not well understood.

Methods

We used shotgun lipidomics in human ccRCC tumors and matched normal adjacent renal tissue. To assess MBOAT7s gene expression across tumor severity, we examined histologically graded human ccRCC samples. We then utilized genome editing in ccRCC cell lines to understand the role of MBOAT7 in ccRCC progression.

Results

We identified a lipid signature for ccRCC that includes an increase in arachidonic acid-enriched phosphat

SUBMITTER: Neumann CKA 

PROVIDER: S-EPMC7033598 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature