LncRNA-PLACT1 sustains activation of NF-κB pathway through a positive feedback loop with IκBα/E2F1 axis in pancreatic cancer.

ABSTRACT:

Background

The activation of NF-κB signaling pathway is regarded as the dominant process that correlates with tumorigenesis. Recently, increasing evidence shows that long noncoding RNAs (lncRNAs) play crucial roles in sustaining the NF-κB signaling pathway. However, the underlying mechanisms have not yet been elucidated.

Methods

The expression and clinical features of PLACT1 were analyzed in a 166-case cohort of PDAC by qRT-PCR and in situ hybridization. The functional role of PLACT1 was evaluated by both in vitro and in vivo experiments. Chromatin isolation by RNA purification assays were utilized to examine the interaction of PLACT1 with IκBα promoter.

Results

We identified a novel lncRNA-PLACT1, which was significantly upregulated in tumor tissues and correlated wi

SUBMITTER: Ren X 

PROVIDER: S-EPMC7033942 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature