Surface-Directed Structural Transition of Amyloidogenic Aggregates and the Resulting Neurotoxicity.

ABSTRACT: The transition of amyloidogenic species into ordered structures (i.e., prefibrillar oligomers, protofibrils, mature fibrils, and amyloidogenic aggregates) is closely associated with many neurodegenerative disease pathologies. It is increasingly appreciated that the liquid-solid interface contributes to peptide aggregation under physiological conditions. However, much remains to be explored on the molecular mechanism of surface-directed amyloid formation. We herein demonstrate that physical environmental conditions (i.e., negatively charged surface) affect amyloid formation. Nontoxic amyloid aggregates quickly develop into intertwisting fibrils on a negatively charged mica surface. These fibrillar structures show significant cytotoxicity on both neuroblastoma cell-lines (SH-SY5Y) and primar

SUBMITTER: Chen H 

PROVIDER: S-EPMC7034003 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature