Novel Fusion Protein Consisting of Metallothionein, Cellulose Binding Module, and Superfolder GFP for Lead Removal from the Water Decoction of Traditional Chinese Medicine.

ABSTRACT: Many methods have been used to detect heavy metals in herbal medicines, while few are developed to remove them. In this study, a novel genetically engineered fusion protein composed of metallothionein (MT), cellulose binding module (CBM), and superfolder GFP (sfGFP) was designed to remove heavy metals. MT, a kind of cysteine-rich protein, was used to chelate heavy metals with high specific affinity. The CBM facilitated the fusion protein MT-CBM-sfGFP binding to cellulose specifically, which made the purification and immobilization in one step. The sfGFP was used to detect the fusion protein MT-CBM-sfGFP easily during the process of expression and immobilization. The MT from Cancer pagurus (MT_Cap) and the CBM from Cellulomonas fimi (CBM_Cef) were used as a

SUBMITTER: Xiao Q 

PROVIDER: S-EPMC7034022 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature