CtBP1-Mediated Membrane Fission Contributes to Effective Recycling of Synaptic Vesicles.

ABSTRACT: Compensatory endocytosis of released synaptic vesicles (SVs) relies on coordinated signaling at the lipid-protein interface. Here, we address the synaptic function of C-terminal binding protein 1 (CtBP1), a ubiquitous regulator of gene expression and membrane trafficking in cultured hippocampal neurons. In the absence of CtBP1, synapses form in greater density and show changes in SV distribution and size. The increased basal neurotransmission and enhanced synaptic depression could be attributed to a higher vesicular release probability and a smaller fraction of release-competent SVs, respectively. Rescue experiments with specifically targeted constructs indicate that, while synaptogenesis and release probability are controlled by nuclear CtBP1, the efficient recycling of SVs relies on its

SUBMITTER: Ivanova D 

PROVIDER: S-EPMC7034063 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature